Transcriptomics

Dataset Information

6

Characterization of IGF1 effects on the transcriptome of normal and irradiated Sox9-EGFP cell populations


ABSTRACT: Transcriptional profiling of intestinal epithelial cells expressing either Negative, Sublow, Low or High levels of the Sox9-EGFP reporter transgene FACS-isolated from jejunum of non-irradiated mice or at 5 days after 14Gy abdominal irradiation. In both groups, mice were treated for 5 consecutive days with either IGF1 or vehicle via mini-pumps (Alzet 1007D, IGF1 at 10mg/ml) implanted subcutaneously immediately following radiation. 4 distinct cell populations FACS-isolated based on levels of expression of the Sox9-EGFP reporter transgene (Sox9-EGFP Negative, Sublow, Low and High cells). 4 conditions: Non-irradiated/vehicle vs Non-irradiated/IGF1 vs Irradiated/vehicle vs Irradiated/IGF1. Biological replicates: 7 independent non-irradiated mice treated with vehicle - 3 independent non-irradiated mice treated for 5 days with IGF1 - 3 independent irradiated mice studied at day 5 post-irradiation treated with vehicle - 3 independent irradiated mice studied at day 5 post-irradiation treated for 5 days with IGF1.

ORGANISM(S): Mus musculus  

SUBMITTER: P. Kay Lund   Laurianne Van Landeghem  P K Lund  Amanda T Mah 

PROVIDER: E-GEOD-42817 | ArrayExpress | 2015-04-14

SECONDARY ACCESSION(S): GSE42817PRJNA183489

REPOSITORIES: GEO, ArrayExpress

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Publications

IGF1 stimulates crypt expansion via differential activation of 2 intestinal stem cell populations.

Van Landeghem Laurianne L   Santoro M Agostina MA   Mah Amanda T AT   Krebs Adrienne E AE   Dehmer Jeffrey J JJ   McNaughton Kirk K KK   Helmrath Michael A MA   Magness Scott T ST   Lund P Kay PK  

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20150402 7


Insulin-like growth factor 1 (IGF1) has potent trophic effects on normal or injured intestinal epithelium, but specific effects on intestinal stem cells (ISCs) are undefined. We used Sox9-enhanced green fluorescent protein (EGFP) reporter mice that permit analyses of both actively cycling ISCs (Sox9-EGFP(Low)) and reserve/facultative ISCs (Sox9-EGFP(High)) to study IGF1 action on ISCs in normal intestine or during crypt regeneration after high-dose radiation-induced injury. We hypothesized that  ...[more]

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