Transcriptomics

Dataset Information

120

Analysis of transcriptional response to mutated Potyvirus Potato virus A that induces necrotic symptoms.


ABSTRACT: Mutations introduced to a highly variable region in HCpro reduced HCpro-HIP2 interactions and virulence of PVA, and induced novel types of necrotic symptoms in the systemically infected leaves of Nicotiana species. Microarray-based analysis of gene expression revealed induction of large numbers of defence-related genes including jasmonic acid and ethylene inducible pathways in the leaves systemically infected by the mutated PVA. Three treaments: infection with wild type PVA (WT), infection with mutated PVA (MUT), and mock-inoculation (Mock). Six plants were treated for each treatment. Experiment was repeated three times. WT and MUT samples from all three experiments were hybridised on microarray. Mock samples from experiments 1 and 2 were hybridised as healthy controls.

ORGANISM(S): Nicotiana tabacum  

SUBMITTER: Tuuli Haikonen   Jari P Valkonen  Yan-Ping Tian  Minna-Liisa Rajamaki 

PROVIDER: E-GEOD-42833 | ArrayExpress | 2013-03-23

SECONDARY ACCESSION(S): GSE42833PRJNA183700

REPOSITORIES: GEO, ArrayExpress

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Publications

Mutation of a Short Variable Region in HCpro Protein of Potato virus A Affects Interactions with a Microtubule-Associated Protein and Induces Necrotic Responses in Tobacco.

Haikonen Tuuli T   Rajamäki Minna-Liisa ML   Tian Yan-Ping YP   Valkonen Jari P T JP  

Molecular plant-microbe interactions : MPMI 20130701 7


Helper component proteinase (HCpro) is a multifunctional protein of potyviruses (genus Potyvirus). HCpro of Potato virus A (PVA) interacts with the microtubule-associated protein HIP2 in host cells, and depletion of HIP2 reduces virus accumulation. This study shows that HCpro of Potato virus Y and Tobacco etch virus also interact with HIP2. The C-proximal portion of PVA HCpro determines the interaction with HIP2 and was found to contain a stretch of six residues comprising a highly variable regi  ...[more]

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