Transcriptomics

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Marker gene discovery for Staphylococcus epidermidis infection in zebrafish embryos (RNA-Seq)


ABSTRACT: We use the zebrafish embryo model to study the innate immune response against Staphylococcus epidermidis. Therefore, we injected S. epidermidis into the yolk at 2 hpf and took samples at 5 days post injection. This deep sequence study was designed to determine the gene expression profile by Staphylococcus epidermidis infection. RNA was isolated from embryos at 5 days post injection. Wildtypes zebrafish embryos were micro-injected into the yolk (2hpf) with 20 CFU of S. epidermdis O-47 mCherry bacteria suspended in PVP (Polyvinylpyrrolidone), or Non-injected as a control. After injections embryos were transferred into fresh egg water and incubated at 28°C. At 5 days post injection 100-200 embryos per group were snap-frozen in liquid nitrogen, and total RNA was isolated using TRIZOL reagent.

ORGANISM(S): Danio rerio  

SUBMITTER: Wouter Veneman   W J Veneman  A H Meijer  H P Spaink 

PROVIDER: E-GEOD-42846 | ArrayExpress | 2013-04-01

SECONDARY ACCESSION(S): SRP017511GSE42846PRJNA183633

REPOSITORIES: GEO, ArrayExpress, ENA

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