Genomics

Dataset Information

309

The enhancer and promoter landscape of human regulatory and conventional T cell subpopulations


ABSTRACT: CD4+CD25+FOXP3+ human regulatory T cells (Treg) are essential for self-tolerance and immune homeostasis. Here, we generated genome-wide maps of poised and active enhancer elements marked by histone H3 lysine 4 monomethylation and histone H3 lysine 27 acetylation for CD4+CD25highCD45RA+ naive and CD4+CD25highCD45RA- memory Treg and their CD25- conventional T cell (Tconv) counterparts after in vitro expansion . In addition we generated genome-wide maps of the transcription factors STAT5, FOXP3, RUNX1 and ETS1 in expanded CD4+CD25highCD45RA+ Treg- and CD4+CD25- Tconv to elucidate their role in cell type-specific gene regulation. ChIP-seq of 2 histone marks and transcription factors ETS1, STAT5, FOXP3 and RUNX1 in expanded T cell subpopulations

ORGANISM(S): Homo sapiens  

SUBMITTER: Michael Rehli   Christian Schmidl 

PROVIDER: E-GEOD-43119 | ArrayExpress | 2014-04-01

SECONDARY ACCESSION(S): SRP017669GSE43119PRJNA185269

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications


CD4(+)CD25(+)FOXP3(+) human regulatory T cells (Tregs) are essential for self-tolerance and immune homeostasis. Here, we describe the promoterome of CD4(+)CD25(high)CD45RA(+) naïve and CD4(+)CD25(high)CD45RA(-) memory Tregs and their CD25(-) conventional T-cell (Tconv) counterparts both before and after in vitro expansion by cap analysis of gene expression (CAGE) adapted to single-molecule sequencing (HeliScopeCAGE). We performed comprehensive comparative digital gene expression analyses and rev  ...[more]

Similar Datasets

2009-05-20 | E-GEOD-14232 | ArrayExpress
| GSE94727 | GEO
2010-05-18 | E-GEOD-14233 | ArrayExpress
2010-05-19 | E-GEOD-14234 | ArrayExpress
2013-06-06 | E-GEOD-30847 | ArrayExpress
2008-12-19 | E-GEOD-6813 | ArrayExpress
2012-02-10 | E-MEXP-2578 | ArrayExpress
2005-01-24 | E-MEXP-228 | ArrayExpress
2014-12-05 | E-GEOD-63876 | ArrayExpress
2015-04-30 | E-GEOD-61834 | ArrayExpress