Dataset Information


Identification of in vivo, conserved, FUS RNA binding sites and the impact of FUS on the neuronal transcriptome

ABSTRACT: FUS, an RNA binding protein was recently implicated in Amyotrophic Lateral Sclerosis (ALS). ALS is a fatal neurodegenerative disease. We report the identification of the conserved neuronal RNA targets of FUS and the assessment of the impact of FUS depletion on the neuronal transcriptome. We identified that FUS regulates splicing of conserved intron containing transcripts. FUS retains or excludes the conserved intron by binding to them. Identification of FUS neuronal targets using normal human brain samples and mouse neurons

ORGANISM(S): Musculus  

SUBMITTER: Alexandra Chang   Panagiotis Alexiou  Manolis Maragkakis  Zissimos Mourelatos  Tadashi Nakaya 

PROVIDER: E-GEOD-43308 | ArrayExpress | 2013-01-25



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FUS regulates genes coding for RNA-binding proteins in neurons by binding to their highly conserved introns.

Nakaya Tadashi T   Alexiou Panagiotis P   Maragkakis Manolis M   Chang Alexandra A   Mourelatos Zissimos Z  

RNA (New York, N.Y.) 20130206 4

Dominant mutations and mislocalization or aggregation of Fused in Sarcoma (FUS), an RNA-binding protein (RBP), cause neuronal degeneration in Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Lobar Degeneration (FTLD), two incurable neurological diseases. However, the function of FUS in neurons is not well understood. To uncover the impact of FUS in the neuronal transcriptome, we used high-throughput sequencing of immunoprecipitated and cross-linked RNA (HITS-CLIP) of FUS in human brains an  ...[more]

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