Transcriptomics

Dataset Information

8

Identifying vitamin C and RA-regulated genes in the J1 mESCs


ABSTRACT: It has been demonstrated that vitamin C enhances reprogramming efficiency during inducing pluripotent stem cells, however, the underlying mechanisms are not fully understood. To find the downstream target genes of vitamin C and investigate the mechanism of vitamin C on reprogramming promotion, we performed Microarray analyses to identify its downstream targets. Retinoic acid (RA), a stimulus molecule for cellular differentiation, is set as negative control. The data show the genes regulated by vitamin C or RA. J1 mESCs maintained in medium containing 1000 U/mL LIF and supplemented without or with vitamin C or RA for 24 hours, then total RNA was extracted for analysis.

ORGANISM(S): Mus musculus  

SUBMITTER: Zhiying Ai   Juan Du  Xiaoyan Shi  Ye Liu  Y GAO  Yongyan Wu  Zekun Guo 

PROVIDER: E-GEOD-43405 | ArrayExpress | 2014-01-21

SECONDARY ACCESSION(S): GSE43405PRJNA185988

REPOSITORIES: GEO, ArrayExpress

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Publications

Vitamin C facilitates pluripotent stem cell maintenance by promoting pluripotency gene transcription.

Gao Yuan Y   Yang Liping L   Chen Linlin L   Wang Xiangxue X   Wu Haibo H   Ai Zhiying Z   Du Juan J   Liu Ye Y   Shi Xiaoyan X   Wu Yongyan Y   Guo Zekun Z   Zhang Yong Y  

Biochimie 20130813 11


Vitamin C has recently received attention because of its ability to improve induced pluripotent stem cells (iPSCs) generation [1-3] and maintain a blastocyst-like state in ES cells [4]. However, the underlying mechanisms are not fully understood. In this study, we found that vitamin C maintained the morphology of mouse embryonic stem cell (mESC) colonies and inhibited mESC differentiation. Gene expression profiling revealed that the genes down-regulated by vitamin C were grouped in the regulatio  ...[more]

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