Transcriptomics

Dataset Information

0

Gene expression of rat PSCs cultured on plastic, matrigel and collagen


ABSTRACT: Activated pancreatic stellate cells produce the fibrotic matrix in chronic pancreatitis and pancreatic cancer. In vitro protocols examining PSC biology have usually involved PSCs cultured on plastic, a non-physiological surface. However, PSCs cultured on physiological matrices e.g. MatrigelTM (normal basement membrane) and collagen (fibrotic pancreas), may have distinctly different behaviours compared to cells cultured on plastic. Therefore, we aimed to compare PSC gene expression after culture on plastic, MatrigelTM and collagen I. Total RNA from stellate cells in 10 cm Petri dishes was isolated by Qiagen RNeasy Mini Plus kit as per manufacturer’s instructions. The Agilent 2100 Bioanalyzer (Agilent Technologies Inc. Santa Clara, CA) was used for quality control of the isolated total RNA. Gene expression profiles of rat PSCs cultured on MatrigelTM, collagen I and plastic were analysed by whole rat genome microarray purchased from Affymetrix (Rat Gene 1.0 ST Array). This array was able to detect 27,342 rat genes, with approximately 26 probes on average per gene (referred to as a probe set).

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Phoebe Phillips  Susan Yang   Zhihong Xu   Minoti Apte    

PROVIDER: E-GEOD-43496 | ArrayExpress | 2013-01-17

SECONDARY ACCESSION(S): GSE43496PRJNA186664

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-10-03 | E-GEOD-43770 | ArrayExpress
| PRJNA186664 | ENA
2013-09-24 | E-GEOD-43730 | ArrayExpress
2012-12-27 | E-GEOD-30407 | ArrayExpress
2014-01-13 | E-GEOD-30406 | ArrayExpress
2012-12-27 | E-GEOD-30405 | ArrayExpress
2015-09-10 | E-GEOD-61305 | ArrayExpress
2015-08-19 | E-GEOD-43730 | ExpressionAtlas
2012-09-05 | E-GEOD-40565 | ArrayExpress
2012-05-10 | E-GEOD-37898 | ArrayExpress