Transcriptomics

Dataset Information

0

Gene expression profiling in cumulus cells (CC) derived from a modified natural and stimulated in vitro fertilization (IVF) procedures


ABSTRACT: Cumulus cells surrounding mature oocytes that developed to moruale/blastocyst stage on day 5 of IVF cycle were collected and used for gene expression profiling using Affymetrix Human Gene 1.0 ST Arrays in order to determine differences in gene expression between the modified natural and stimulated in vitro fertilization (IVF) procedures. Microarray analysis was made on 8 individual CC samples, derived from 5 patients that first underwent MNIVF, followed by a controlled ovarian hyperstimulation (COH) cycle. As the quality of the isolated RNA derived from 2 of the MNIVF CC samples was not good, these samples were not included in the analysis. Altogether, 3 CC samples from MNIVF and 5 CC samples from COH cycles were included in microarray analysis.

ORGANISM(S): Homo sapiens  

SUBMITTER: Peter Juvan  Tanja Burnik Papler   Irma Virant Klun   Klementina Fon Tacer   Rok Devjak   Eda Vrtačnik Bokal    

PROVIDER: E-GEOD-43684 | ArrayExpress | 2014-01-10

SECONDARY ACCESSION(S): GSE43684PRJNA187053

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Differences in cumulus cells gene expression between modified natural and stimulated in vitro fertilization cycles.

Papler Tanja Burnik TB   Bokal Eda Vrtačnik EV   Tacer Klementina Fon KF   Juvan Peter P   Virant Klun Irma I   Devjak Rok R  

Journal of assisted reproduction and genetics 20131113 1


PURPOSE: The aim of our study was to determine whether there are any differences in the cumulus cell gene expression profile of mature oocytes derived from modified natural IVF and controlled ovarian hyperstimulation cycles and if these changes could help us understand why modified natural IVF has lower success rates. METHODS: Cumulus cells surrounding mature oocytes that developed to morulae or blastocysts on day 5 after oocyte retrieval were submitted to microarray analysis. The obtained data  ...[more]

Similar Datasets

2013-09-13 | E-GEOD-34230 | ArrayExpress
2008-10-21 | E-GEOD-12034 | ArrayExpress
2009-12-31 | E-MEXP-2347 | ArrayExpress
2018-05-24 | PXD006641 | Pride
2012-11-06 | E-GEOD-34526 | ArrayExpress
2014-02-15 | E-MTAB-2203 | ArrayExpress
2016-08-01 | E-GEOD-80289 | ArrayExpress
2013-06-05 | E-GEOD-40400 | ArrayExpress
2016-08-11 | E-GEOD-85436 | ArrayExpress
2016-08-11 | E-GEOD-85438 | ArrayExpress