Dataset Information


Identification of genes responsive to mild hyperthermia in human cervical squamous cell carcinoma HeLa cells

ABSTRACT: Hyperthermia (HT) is widely used to treat patients with various cancers. In general, HT elicits a wide spectrum of stress responses such as induction of heat shock proteins, protein aggregation and cell death in mammalian cells. Although many biological processes are affected by HT, the overall responses to HT in mammalian cells remain unknown. The effects of heat stress at 41°C for 30 min (mild hyperthermia) on the gene expression in human cervical squamous cell carcinoma HeLa cells were investigated using an Affymetrix GeneChip system. Human cervical squamous cell carcinoma HeLa cells were treated with heat stress (41°C for 30 min) and followed by incubation for 0, 1, or 3 h at 37°C. Non-treated cells were served as control. Total RNA samples were prepared from the cells, and quality of the RNA was analyzed using a Bioanalyzer 2100. Gene expression was monitored by an Affymetrix GeneChip® system with a Human Genome U133-plus 2.0 array. Sample preparation for array hybridization was carried out as described in the manufacturer’s instructions.

ORGANISM(S): Homo sapiens  

SUBMITTER: Yoshiaki Tabuchi   Tatsuya Yunoki  Ayako Kariya 

PROVIDER: E-GEOD-43701 | ArrayExpress | 2013-01-24



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Identification of common gene networks responsive to mild hyperthermia in human cancer cells.

Kariya Ayako A   Tabuchi Yoshiaki Y   Yunoki Tatsuya T   Kondo Takashi T  

International journal of molecular medicine 20130430 1

Hyperthermia (HT) has been used as a possible treatment modality for various types of malignant tumors. Due to its pleiotropic effects, its combined use with radiotherapy and/or chemotherapy has proven to be beneficial. However, the molecular mechanisms underling the cellular responses to heat stress remain unclear. Therefore, the aim of this study was to identify common gene expression patterns responsive to mild HT (MHT) in human cancer cells. HeLa human cervical squamous cell carcinoma (SCC)  ...[more]

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