Transcriptomics

Dataset Information

4

Campylobacter jejuni Cj1000 regulon


ABSTRACT: Transcriptional regulation mediates adaptation of pathogens to environmental stimuli and is important for host colonisation. The Campylobacter jejuni genome sequence reveals a surprisingly small set of regulators, mostly of unknown function, suggesting an intricate regulatory network. Interestingly, C. jejuni lacks the homologues of ubiquitous regulators involved in stress response found in many other Gram-negative bacteria. Nonetheless, cj1000 is predicted to code for the sole LysR-type regulator in the C. jejuni genome, and thus may be involved in major adaptation pathways. A cj1000 mutant strain was constructed and found to be attenuated in its ability to colonise 1-day old chicks. Complementation of cj1000 mutation restored the colonisation ability to that of wild type levels. The mutant strain was also outcompeted in a competitive colonisation assay of the piglet intestine. High resolution oxygraphy was carried out for the first time on C. jejuni and revealed a role for Cj1000 in controlling O2 consumption. Furthermore, microarray analysis of the cj1000 mutant revealed both direct and indirect regulatory targets, including genes involved in energy metabolism and oxidative stress defences. These results highlight the importance of Cj1000 regulation in host colonisation and in major physiological pathways. Microarray data was collected from three independent biological replicates and 3-9 technical replicates for each biological replicate.

ORGANISM(S): Campylobacter jejuni  

SUBMITTER: Alain Stintzi   Annika Flint  Jennifer Li  Virginie Dufour  Christine Baysse 

PROVIDER: E-GEOD-43792 | ArrayExpress | 2013-07-03

SECONDARY ACCESSION(S): GSE43792PRJNA187449

REPOSITORIES: GEO, ArrayExpress

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Publications

Inactivation of the LysR regulator Cj1000 of Campylobacter jejuni affects host colonization and respiration.

Dufour Virginie V   Li Jennifer J   Flint Annika A   Rosenfeld Eric E   Rivoal Katell K   Georgeault Sylvie S   Alazzam Bachar B   Ermel Gwennola G   Stintzi Alain A   Bonnaure-Mallet Martine M   Baysse Christine C  

Microbiology (Reading, England) 20130404 Pt 6


Transcriptional regulation mediates adaptation of pathogens to environmental stimuli and is important for host colonization. The Campylobacter jejuni genome sequence reveals a surprisingly small set of regulators, mostly of unknown function, suggesting an intricate regulatory network. Interestingly, C. jejuni lacks the homologues of ubiquitous regulators involved in stress response found in many other Gram-negative bacteria. Nonetheless, cj1000 is predicted to encode the sole LysR-type regulator  ...[more]

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