Transcriptomics

Dataset Information

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Human glioma cell line T98G: BAP-PCBP2 RIP vs. BAP-GFP RIP (Control)


ABSTRACT: RIP-Chip analysis of PCBP2 and identification of preferentially associated mRNAs. T98G cells were transfected transiently with BAP-tagged constructs. BAP-tagged proteins were biotinylated in vivo by the co-transfected hBirA enzyme. RNPs were recovered via precipitation with Steptavidin-sepharose beads. Finally, RNAs were purified and analyzed on microarrays. BAP-GFP as control was used in three independent sets of experiments. Two-condition experiment, BAP-PCBP2 vs. BAP-GFP cells. Biological replicates: 3 BAP-PCBP2 replicates, 3 BAP-GFP (Control) replicates

ORGANISM(S): Homo sapiens  

SUBMITTER: zhong xiao peng   Wei Han 

PROVIDER: E-GEOD-44048 | ArrayExpress | 2013-07-24

SECONDARY ACCESSION(S): GSE44048PRJNA188445

REPOSITORIES: GEO, ArrayExpress

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Publications

RNA-binding protein PCBP2 modulates glioma growth by regulating FHL3.

Han Wei W   Xin Zhongshuai Z   Zhao Zhiqiang Z   Bao Wen W   Lin Xihua X   Yin Bin B   Zhao Jizong J   Yuan Jiangang J   Qiang Boqin B   Peng Xiaozhong X  

The Journal of clinical investigation 20130415 5


PCBP2 is a member of the poly(C)-binding protein (PCBP) family, which plays an important role in posttranscriptional and translational regulation by interacting with single-stranded poly(C) motifs in target mRNAs. Several PCBP family members have been reported to be involved in human malignancies. Here, we show that PCBP2 is upregulated in human glioma tissues and cell lines. Knockdown of PCBP2 inhibited glioma growth in vitro and in vivo through inhibition of cell-cycle progression and inductio  ...[more]

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