Transcriptomics

Dataset Information

7

Genome-wide analysis of host mRNA translation during hepatitis C virus infection


ABSTRACT: Sucrose gradient ultracentrifugation followed by microarray analysis was used to identify translationally-regulated mRNAs (mRNAs associated with ribosomes) from JFH1-infected and uninfected Huh-7.5.1 cells. Huh7 cells were infected with HCV JFH-1 strain. Controls consisted in non-infected cells and infected cells treated with an anti-protease. 3 days after infection, cell lysates were subjected to sucrose-gradient centrifugation in order to isolate polysome-free RNAs from polysome-bound (translated) RNAs. The non-polysomal, polysomal as well as cytosolic (non fractionned) RNAs, from infected, non-infected, and infected-treated cells were subject sur microarray analysis.

ORGANISM(S): Homo sapiens  

SUBMITTER: Sandra Pierredon   Catherine Le Berre-Scoul  Cyrille Féray  Helene Colman  Céline Hernandez  Audrey Bihouée  Stephan Vagner  Arielle R Rosenberg  Rémi Houlgatte  Hélène Colman 

PROVIDER: E-GEOD-44210 | ArrayExpress | 2013-02-12

SECONDARY ACCESSION(S): GSE44210PRJNA189181

REPOSITORIES: GEO, ArrayExpress

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