Genomics

Dataset Information

3

Master Transcription Factors and Mediator Establish Super-Enhancers at Key Cell Identity Genes [ChIP-Seq]


ABSTRACT: The master transcription factors Oct4, Sox2 and Nanog bind enhancer elements and recruit the Mediator co-activator to activate much of the gene expression program of embryonic stem cells (ESCs). We report here that the ESC master transcription factors and Mediator form “super-enhancers” at most genes known to control the pluripotent state, including those encoding the master transcription factors themselves. These super-enhancers consist of extraordinarily large genomic domains occupied by exceptional amounts of Oct4, Sox2, Nanog, Klf4, Esrrb and Mediator. Super-enhancers stimulate considerably higher transcription than typical enhancers in vivo and in reporter vectors. Reduced levels of Oct4 or Mediator cause preferential loss of expression of super-enhancer-associated genes relative to other genes, suggesting how changes in gene expression programs might be accomplished during development. In other more differentiated cells, super-enhancers containing cell-type-specific master transcription factors are also found at genes that define cell identity. These results implicate super-enhancers in the control of mammalian cell identity and differentiation. ChIP-Seq and controls associated with Super-Enhancers in murine cell types

ORGANISM(S): Mus musculus  

SUBMITTER: Jessica Reddy   Warren Whyte  Brian J Abraham  Jamie J Newman  David Orlando  Denes Hnisz  Steve Bilodeau  Richard A Young  Alan C Mullen 

PROVIDER: E-GEOD-44286 | ArrayExpress | 2013-02-13

SECONDARY ACCESSION(S): SRP018619GSE44286PRJNA189323

REPOSITORIES: GEO, ArrayExpress, ENA

Dataset's files

Source:
Action DRS
E-GEOD-44286.idf.txt Idf
E-GEOD-44286.processed.1.zip Processed
E-GEOD-44286.processed.2.zip Processed
E-GEOD-44286.processed.3.zip Processed
E-GEOD-44286.sdrf.txt Txt
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