Genomics

Dataset Information

300

Whole-genome landscape of histone H2AX and γ-H2AX along with related factors in activated cells


ABSTRACT: Phosphorylation of the histone variant H2AX forms γ-H2AX, which serves as a marker of DNA repair response. Here we provide ChIP-seq-based maps of histone H2AX, γ-H2AX, H2AZ, INO80, SRCAP, and RNA polymerase II in activated T cells. Matched data for H2AX and γ-H2AX in resting T cells and Jurkat cancer T cells are available in GSE25577. CD4+ T cells were stimulated in two different ways (IL-2 alone or IL-2 plus anti-CD3 and anti-CD28), and H2AX, γ-H2AX, H2AZ, INO80, and SRCAP profiles were examined by ChIP-seq

ORGANISM(S): Homo sapiens  

SUBMITTER: Jung K Choi   Jungmin Seo 

PROVIDER: E-GEOD-44309 | ArrayExpress | 2014-04-01

SECONDARY ACCESSION(S): SRP018668GSE44309PRJNA189444

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Genome-wide reorganization of histone H2AX toward particular fragile sites on cell activation.

Seo Jungmin J   Kim Kwoneel K   Chang Dong-Yeop DY   Kang Ho-Bum HB   Shin Eui-Cheol EC   Kwon Jongbum J   Choi Jung Kyoon JK  

Nucleic Acids Research 20131024 2


γH2AX formation by phosphorylation of the histone variant H2AX is the key process in the repair of DNA lesions including those arising at fragile sites under replication stress. Here we demonstrate that H2AX is dynamically reorganized to preoccupy γH2AX hotspots on increased replication stress by activated cell proliferation and that H2AX is enriched in aphidicolin-induced replisome stalling sites in cycling cells. Interestingly, H2AX enrichment was particularly found in genomic regions that rep  ...[more]

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