Genomics

Dataset Information

300

Gene expression is a circular process


ABSTRACT: This study involves the role of yeast mRNA decay factors in transcription. The experiment included here are the ChIP-exo results of three decay factors: Xrn1, Dcp2 & Lsm1. Four experiments were made: Xrn1, Dcp2, Lsm1 and control (no-TAP tag), in two replicates.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Mordechai Choder   Manuel Garber  Gal Haimovich 

PROVIDER: E-GEOD-44312 | ArrayExpress | 2013-05-23

SECONDARY ACCESSION(S): GSE44312SRP018648PRJNA189422

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

ChIP-exo method for identifying genomic location of DNA-binding proteins with near-single-nucleotide accuracy.

Rhee Ho Sung HS   Pugh B Franklin BF  

Current protocols in molecular biology 20121001


This unit describes the ChIP-exo methodology, which combines chromatin immunoprecipitation (ChIP) with lambda exonuclease digestion followed by high-throughput sequencing. ChIP-exo allows identification of a nearly complete set of the binding locations of DNA-binding proteins at near-single-nucleotide resolution with almost no background. The process is initiated by cross-linking DNA and associated proteins. Chromatin is then isolated from nuclei and subjected to sonication. Subsequently, an ant  ...[more]

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