Genomics

Dataset Information

358

Whole genome bisulfite sequencing of rdm16ros1, ros1, nrpd1ros1 mutants and C24 wild-type


ABSTRACT: Using whole genome bisulfite sequencing to provide single-base resulution of DNA methylation status in rdm16ros1, ros1, nrpd1ros1 mutants and examine the effect of RDM16 on DNA methylation 4 samples examined: C24 wild type with RD29A-LUC transgene, rdm16ros1 double mutant, ros1 mutant, nrpd1ros1 mutant (all in C24 background with RD29A-LUC transgene)

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Renyi Liu   Zeyang Ma  Xinjian He  Daisuke Miki  Heng Zhang  Wei Chen  Jian-Kang Zhu  Chao-Feng Huang  Kai Tang  Lan Yang  Zhimin Zheng 

PROVIDER: E-GEOD-44417 | ArrayExpress | 2013-09-27

SECONDARY ACCESSION(S): SRP018768GSE44417PRJNA189938

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

A Pre-mRNA-splicing factor is required for RNA-directed DNA methylation in Arabidopsis.

Huang Chao-Feng CF   Miki Daisuke D   Tang Kai K   Zhou Hao-Ran HR   Zheng Zhimin Z   Chen Wei W   Ma Ze-Yang ZY   Yang Lan L   Zhang Heng H   Liu Renyi R   He Xin-Jian XJ   Zhu Jian-Kang JK  

PLoS genetics 20130912 9


Cytosine DNA methylation is a stable epigenetic mark that is frequently associated with the silencing of genes and transposable elements (TEs). In Arabidopsis, the establishment of DNA methylation is through the RNA-directed DNA methylation (RdDM) pathway. Here, we report the identification and characterization of RDM16, a new factor in the RdDM pathway. Mutation of RDM16 reduced the DNA methylation levels and partially released the silencing of a reporter gene as well as some endogenous genomic  ...[more]

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