Transcriptomics

Dataset Information

3

Androgen receptor-coregulators hormone responsiveness LNCaP / GSF


ABSTRACT: The text description is: LNCaP are androgen receptor expressing prostate carcinoma cells. Genital skin fibroblasts also express the androgen receptor. Cells were either proliferating or they were G0-arrested. Treatment of cells was performed with either the androgen DHT (dihydrotestosterone), the androgen analogue R1881 (methyltrienolone), or the solvent ethanol. Some hybridizations were performed in the type 1 design. In these cases, the hormone treated sample was hybridized against the ethanol treated sample on the same microarray. Hormone mediated induction or repression of gene transcription can directly be deduced from R/G normalized ratios on arrays. For other experiments, the ethanol treated control and the hormone treated experiment were hybridized on separate microarrays against a common reference of RNA. This reference was composed out of common reference batch CRG (50%) and a fibroblast RNA (50%). This reference was called mixed reference. For all experiments, the same batch of mixed reference was used. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: Ethanol, R1881 (methyltrienolone), or DHT (dihydrotestosterone) Cell Line: LNCaP (prostate cancer) or foreskin fibroblasts Culture Synchrony: proliferation / Go-arrest Computed

ORGANISM(S): Homo sapiens  

SUBMITTER: Samuel DePrimo   Paul-Martin Holterhus 

PROVIDER: E-GEOD-4454 | ArrayExpress | 2010-07-01

SECONDARY ACCESSION(S): GSE4454PRJNA104675

REPOSITORIES: GEO, ArrayExpress

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Publications

Cell-line and tissue-specific signatures of androgen receptor-coregulator transcription.

Bebermeier Jan-Hendrik JH   Brooks James D JD   DePrimo Samuel E SE   Werner Ralf R   Deppe Uta U   Demeter Janos J   Hiort Olaf O   Holterhus Paul-Martin PM  

Journal of molecular medicine (Berlin, Germany) 20060824 11


Normal genital skin fibroblasts (GSF) and the human prostate carcinoma cell line LNCaP have been used widely as cell culture models of genital origin to study androgen receptor (AR) signaling. We demonstrate that LNCaP shows a reproducible response to androgens as assessed using cDNA-microarrays representing approximately 32,000 unique human genes, whereas several independent GSF strains are virtually unresponsive. We show that LNCaP cells express markedly higher AR protein levels likely contrib  ...[more]

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