Dataset Information


Expression data from C2C12 myotubes infected with RML prions

ABSTRACT: Prion infection in animals results in neurodegeneration and eventually death. To examine the cellular impact of Prion disease, we profiled non-proliferative fully differentiated C2C12 cells, which can replicate prions to high levels. Results suggest that accumulation of high levels of PrPSc in C2C12 myotubes does not cause any overt cellular dysfunction or molecular pathology. C2C12 cells were differentiated into confluent myotubes. Cells were infected or not with 100ul of 10% brain homogenate obtained from a C57BL/6 mouse clinically affected with RML prions. 16 days after infection, cells were collected by scraping and RML was purified.


ORGANISM(S): Mus musculus  

SUBMITTER: Debbie McKenzie   Allen Herbst 

PROVIDER: E-GEOD-44563 | ArrayExpress | 2013-12-22



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Infectious prions accumulate to high levels in non proliferative C2C12 myotubes.

Herbst Allen A   Banser Pamela P   Velasquez Camilo Duque CD   Mays Charles E CE   Sim Valerie L VL   Westaway David D   Aiken Judd M JM   McKenzie Debbie D  

PLoS pathogens 20131107 11

Prion diseases are driven by the strain-specific, template-dependent transconformation of the normal cellular prion protein (PrP(C)) into a disease specific isoform PrP(Sc). Cell culture models of prion infection generally use replicating cells resulting in lower levels of prion accumulation compared to animals. Using non-replicating cells allows the accumulation of higher levels of PrP(Sc) and, thus, greater amounts of infectivity. Here, we infect non-proliferating muscle fiber myotube cultures  ...[more]

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