Genomics

Dataset Information

300

Genome-wide maps of CENP-A nucleosomes in three neocentromere-containing cell lines


ABSTRACT: We used native ChIP-seq of CENP-A-containing particles from normal centromeres on alpha-satellite DNA and three naturally-occurring neocentromeres to test the proposed models for the major form of the fundamental repeating unit of centromeric chromatin. We found that the predominant form of the CENP-A particle at the centromere is an octameric nucleosome with loose terminal DNA. Additionally, we found CENP-A nucleosomes are strongly phased on the 171 bp alpha-satellite monomers of normal centromeres, and also display strong positioning and neocentromeres. Comparison of CENP-A and bulk nucleosome DNA lengths and positions in three different human neocentromere-containing cell lines

ORGANISM(S): Homo sapiens  

SUBMITTER: Ben Black  

PROVIDER: E-GEOD-44724 | ArrayExpress | 2013-05-04

SECONDARY ACCESSION(S): SRP018885GSE44724PRJNA191094

REPOSITORIES: GEO, ArrayExpress, ENA

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