Transcriptomics

Dataset Information

2

Identification of the chemokine CCL28 as a growth and survival factor for human hematopoietic stem- and progenitor cells


ABSTRACT: To discover novel growth factors for hematopoietic stem- and progenitor cells (HSPCs), we have assessed cytokine responses of cord blood (CB)-derived CD34+ cells in a high-content growth factor screen. We identify the immunoregulatory chemokine (C-C motif) ligand 28 (CCL28) as a novel growth factor that directly stimulates proliferation of primitive hematopoietic cells from different ontogenetic origins. Microarrays are used to compare the expression profiles of HSPCs expanded in SCF, TPO and CCL28 respectively. HSPCs were cultured in Serum-Free Expansion Medium with SCF, SCF + TPO, and SCF + CCL28 respectively, and hybridised microarrays in triplicate.

ORGANISM(S): Homo sapiens  

SUBMITTER: Shamit Soneji   S Soneji  C Karlsson  L Larsson 

PROVIDER: E-GEOD-45136 | ArrayExpress | 2013-06-03

SECONDARY ACCESSION(S): GSE45136PRJNA192950

REPOSITORIES: GEO, ArrayExpress

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Publications

Identification of the chemokine CCL28 as a growth and survival factor for human hematopoietic stem and progenitor cells.

Karlsson Christine C   Baudet Aurélie A   Miharada Natsumi N   Soneji Shamit S   Gupta Rajeev R   Magnusson Mattias M   Enver Tariq T   Karlsson Göran G   Larsson Jonas J  

Blood 20130318 19


In an attempt to discover novel growth factors for hematopoietic stem and progenitor cells (HSPCs), we have assessed cytokine responses of cord blood (CB)-derived CD34(+) cells in a high-content growth factor screen. We identify the immunoregulatory chemokine (C-C motif) ligand 28 (CCL28) as a novel growth factor that directly stimulates proliferation of primitive hematopoietic cells from different ontogenetic origins. CCL28 enhances the functional progenitor cell content of cultured cells by st  ...[more]

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