Transcriptomics

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Microarray analysis of med14 and wild type (Col-0) infected with the avirulent bacterial pathogen Pst DC3000/avrRpt2


ABSTRACT: The goal of the microarray experiment was to determine the induction kinetics of transcriptome changes and to identify differentially expressed genes in the Arabidopsis mutant med14 and wild type in response to infection of the avirulent bacterial pathogen Pst DC3000/avrRpt2. Results indicated that med14 exhibited slower kinetcis of transcriptional changes than the wild type after Pst DC3000/avrRpt2 infection, and the induction of a large group of defense genes was suppressed in the med14 mutant. Three biological replicates with leaves from 8 plants per sample were collected at 0, 4, 8, and 12 hours after inoculation with the avirulent bacterial pathogen Pst DC3000/avrRpt2. After extraction, RNA concentration was determined on a NanoDrop Spectrophotometer (Thermofisher Scientific, Waltham, MA) and sample quality was assessed using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Each replicate was used as one sample for microarray analysis.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Yijun Sun  Jin Yao   Yanping Zhang   Zhonglin Mou   Xudong Zhang    

PROVIDER: E-GEOD-45212 | ArrayExpress | 2013-05-18

SECONDARY ACCESSION(S): GSE45212PRJNA193214

REPOSITORIES: GEO, ArrayExpress

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