Transcriptomics

Dataset Information

7

Pneumonia infection, lung and liver inflammation


ABSTRACT: Vaccination reduces morbidity and mortality from pneumonia but its effect on the tissue-level response to infection is still poorly understood. We evaluated pneumonia disease progression, acute phase response and lung gene expression profiles in mice inoculated intranasally with virulent gram-positive Streptococcus pneumoniae serotype (ST) 3, with and without prior immunization with pneumococcal polysaccharide ST 3 (PPS3), or co-immunization with PPS3 and with a low dose of lipopolysaccharide (LPS). Pneumonia severity was assessed in the acute phase, 5, 12, 24 and 48 h post-inoculation (p.i.) and the resolution phase of 7 days p.i. Primary PPS3 specific antibody production was upregulated and IgM binding to pneumococci increased in PPS3-immunized mice. Immunizations with PPS3 or PPS3 + LPS decreased bacterial recovery the lung and blood at 24 and 48 h and increased survival. Microarray analysis of whole lung RNA revealed significant changes in the acute phase protein serum amyloid A (SAA) between noninfected and infected mice, which were attenuated by immunization. SAA transcripts were higher in the liver and lungs of infected controls, and SAA protein was elevated in serum, but decreased in PPS3-immunized mice. Thus, during a virulent pneumonia infection, prior immunization with PPS3 in an IgM-dependent manner as well as co-immunization with PPS3 + LPS attenuated pneumonia severity and promoted resolution of infection, concomitant with significant regulation of cytokine gene expression in the lungs, and acute phase proteins in the lungs, liver and serum. Each lung RNA sample represented an individual mouse, creating biological repeats for each treatment. In-vivo treatments were as follows: non-infected lung (vehicle-immunized) control (n=5), infected lung (vehicle-immunized) control at 48 hr post-inoculation (n=5), PPS3 and LPS co-immunized lung at 48 hr post-inoculation (n=4) and PPS3 and LPS co-immunized lung at 7 days post-inoculation (n=4).

ORGANISM(S): Mus musculus  

SUBMITTER: Catharine Ross   A. Catharine Ross  Katherine H Restori 

PROVIDER: E-GEOD-45644 | ArrayExpress | 2013-03-29

SECONDARY ACCESSION(S): GSE45644PRJNA195466

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Immunization with pneumococcal polysaccharide serotype 3 and lipopolysaccharide modulates lung and liver inflammation during a virulent Streptococcus pneumoniae infection in mice.

Restori Katherine H KH   Kennett Mary J MJ   Ross A Catharine AC  

Clinical and vaccine immunology : CVI 20130206 5


Vaccination reduces morbidity and mortality from pneumonia, but its effect on the tissue-level response to infection is still poorly understood. We evaluated pneumonia disease progression, acute-phase response, and lung gene expression profiles in mice inoculated intranasally with virulent Gram-positive Streptococcus pneumoniae serotype 3 (ST 3) with and without prior immunization with pneumococcal polysaccharide ST 3 (PPS3) or after coimmunization with PPS3 and a low dose of lipopolysaccharide  ...[more]

Similar Datasets

2019-07-11 | PXD014070 | Pride
| GSE85336 | GEO
| GSE81219 | GEO
2007-06-09 | GSE7768 | GEO
2008-06-15 | E-GEOD-7768 | ArrayExpress
2010-08-24 | GSE23713 | GEO
2010-08-24 | E-GEOD-23713 | ArrayExpress
2019-03-22 | PXD004520 | Pride
2013-08-18 | E-MTAB-1772 | ArrayExpress
2012-09-15 | E-GEOD-40885 | ArrayExpress