Transcriptomics

Dataset Information

5

Microarray analysis of WT and Notch2-deficient classical DC subsets


ABSTRACT: Defense against attaching and effacing (A/E) bacteria requires the sequential generation of IL-23 and IL-22 to induce protective mucosal responses. While the critical source of IL-22 has been identified as CD4+ and Nkp46+ innate lymphoid cells (ILCs), the precise source of IL-23 is unclear. Here, we use genetic techniques to deplete specific classical dendritic cell (cDC) subsets and analyze immunity to the A/E pathogen Citrobacter rodentium. We find that Zbtb46+ cDCs, and specifically Notch2-dependent intestinal CD11b+ cDCs, but not Batf3-dependent CD103+ cDCs, are required for IL-23 production and immunity against C. rodentium. Notch2 controls cDC differentiation at a terminal step mediated by lymphotoxin signaling. Importantly, these results provide the first demonstration of a non-redundant function of CD11b+ cDCs in vivo. Analysis of Notch2-dependent genes in CD11b+ and DEC205+ splenic classical DC subsets. Splenocytes were harvested from littermate WT Notch2 f/f C57Bl/6 or Notch2 CD11c-cre C57Bl/6 mice and DC subsets sorted to >95% purity on the FACSAriaII.

ORGANISM(S): Mus musculus  

SUBMITTER: Kenneth Murphy   Ansuman Satpathy 

PROVIDER: E-GEOD-45681 | ArrayExpress | 2013-06-01

SECONDARY ACCESSION(S): GSE45681PRJNA195895

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2013-06-01 | E-GEOD-45679 | ArrayExpress
2013-06-01 | E-GEOD-45682 | ArrayExpress
2013-01-01 | S-EPMC3788683 | BioStudies
| GSE45681 | GEO
2016-03-28 | E-GEOD-77304 | ArrayExpress
2012-05-20 | E-GEOD-37028 | ArrayExpress
2015-05-19 | E-GEOD-68590 | ArrayExpress
2016-03-28 | E-GEOD-77303 | ArrayExpress
2013-06-01 | E-GEOD-45698 | ArrayExpress
2014-01-01 | E-GEOD-52857 | ArrayExpress