Transcriptomics

Dataset Information

3

RNA Expression Data from IL-15-/- and IL-15+/+ TAX-LUC Mouse Tumors


ABSTRACT: IL-15 is recognized as a promising candidate for tumor immunotherapy and has been described as both a promoter of cancer and a promoter of anti-cancer immunity. IL-15 was discovered in cells transformed by HTLV-1, the etiologic agent of adult T cell leukemia / lymphoma (ATL) and the human retrovirus that carries the Tax oncogene. We have developed the TAX-LUC mouse model of ATL in which Tax expression drives both malignant transformation and luciferase expression, enabling non-invasive imaging of tumorigenesis in real time. To identify the role of IL-15 in spontaneous development of lymphoma in vivo, an IL-15-/- TAX-LUC strain was developed and examined. The absence of IL-15 resulted in aggressive tumor growth and accelerated mortality and demonstrated that IL-15 was not required for Tax-mediated lymphoma but was essential for anti-tumor immunity. Further analysis revealed a unique transcriptional profile in tumor cells that arise in the absence of IL-15 that included a significant increase in the expression of IL-1α and IL-1α-regulated cytokines. Moreover, anti-IL-1α antibodies and an IL-1 receptor antagonist (Anakinra) were used to interrogate the potential of IL-1α targeted therapies in this model. Taken together, these findings identify IL-15 and IL-1α as therapeutic targets in lymphoma. We used microarrays to compare the gene expression profile of tumors in IL-15-/- TAX-LUC mice to IL-15+/+ TAX-LUC mice RNA was obtained from CD16/32HI and CD16/32LO cells harvested from n=2 IL-15+/+ (control)and n=2 IL-15-/- Tax tumors and was compared to look for alterations in gene expression in malignant and tumor infiltrating cells resulting from loss of IL-15 in vivo

ORGANISM(S): Mus musculus  

SUBMITTER: Daniel Rauch   Rekha Meyer  Lee Ratner 

PROVIDER: E-GEOD-46072 | ArrayExpress | 2013-06-11

SECONDARY ACCESSION(S): GSE46072PRJNA197152

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2014-01-01 | S-EPMC3885672 | BioStudies
1000-01-01 | S-EPMC4603455 | BioStudies
2004-01-01 | S-EPMC416520 | BioStudies
1000-01-01 | S-EPMC3056641 | BioStudies
1000-01-01 | S-EPMC2974607 | BioStudies
2020-01-01 | S-EPMC7067701 | BioStudies
2020-01-01 | S-EPMC7248178 | BioStudies
2009-07-28 | GSE17341 | GEO
2017-01-01 | S-EPMC5642476 | BioStudies
1000-01-01 | S-EPMC4207800 | BioStudies