Transcriptomics

Dataset Information

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Studies on progenitor endothelial cells; exploring mechanisms for improvement of cardiovascular diseases


ABSTRACT: Transcriptomic analysis of primary CD34+ cells. CD34+ cell were induced in vitro with hypoxia (3 hours), high glucose and high glucose plus hypoxia. Subsequently, the effect of metformin (anti-diabetic drug) on all conditions was studied to take advantage of transcriptomics to prospectively explore the mechanism of this drug to reduce the risk of cardiovascular diseases in type II diabetic patients. Total RNA isolated from 20 samples; 10 different conditions each has 2 repeats; labeled, and hybridized to Affymetrix Human Gene 1.0 ST Array.

ORGANISM(S): Homo sapiens  

SUBMITTER: Jayapal Manikandan   Hans-Juergen Schulten  Farid Ahmed  Mohammed H Alqahtani  Sahira Lary  Iokim Spyridopoulos  Jolanta Weaver  Sherin Bakhashab 

PROVIDER: E-GEOD-46262 | ArrayExpress | 2016-02-24

SECONDARY ACCESSION(S): GSE46262PRJNA198490

REPOSITORIES: GEO, ArrayExpress

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Publications

Metformin improves the angiogenic potential of human CD34⁺ cells co-incident with downregulating CXCL10 and TIMP1 gene expression and increasing VEGFA under hyperglycemia and hypoxia within a therapeutic window for myocardial infarction.

Bakhashab Sherin S   Ahmed Fahad W FW   Schulten Hans-Juergen HJ   Bashir Ayat A   Karim Sajjad S   Al-Malki Abdulrahman L AL   Gari Mamdooh A MA   Abuzenadah Adel M AM   Chaudhary Adeel G AG   Alqahtani Mohammed H MH   Lary Sahira S   Ahmed Farid F   Weaver Jolanta U JU  

Cardiovascular diabetology 20160209


Cardiovascular disease (CVD) is the leading cause of morbidity and mortality in patients with diabetes mellitus (DM). To identify the most effective treatment for CVD, it is paramount to understand the mechanism behind cardioprotective therapies. Although metformin has been shown to reduce CVD in Type-2 DM clinical trials, the underlying mechanism remains unexplored. CD34(+) cell-based therapies offer a new treatment approach to CVD. The aim of this study was to investigate the effect of metform  ...[more]

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