Transcriptomics

Dataset Information

416

Small RNA sequencing of human preovulatory cumulus and mural granulosa cells


ABSTRACT: The granulosa cells in the mammalian ovarian follicle respond to gonadotropin signalling and are involved in the processes of folliculogenesis and oocyte maturation. Studies on gene expression and regulation in human granulosa cells are of interest due to their potential for estimating the oocyte viability and IVF success. However, the post-transcriptional gene expression studies on miRNA level in the human ovary have been scarce. The current study determined the miRNA profile by deep sequencing of the two intrafollicular somatic cell types: mural and cumulus granulosa cells isolated from women undergoing controlled ovarian stimulation and in vitro fertilization. Paired cumulus and mural granulosa samples were analysed from 3 women participating in IVF procedure. Libraries of all 6 samples were sequenced twice, generating 2 technical replicates for each sample. Differential gene expression study was performed on the pooled results of technical replicates.

ORGANISM(S): Homo sapiens  

SUBMITTER: Jaak Simm   Agne Velthut-Meikas 

PROVIDER: E-GEOD-46489 | ArrayExpress | 2013-08-05

SECONDARY ACCESSION(S): GSE46489SRP021911PRJNA200699

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Research resource: small RNA-seq of human granulosa cells reveals miRNAs in FSHR and aromatase genes.

Velthut-Meikas Agne A   Simm Jaak J   Tuuri Timo T   Tapanainen Juha S JS   Metsis Madis M   Salumets Andres A  

Molecular endocrinology (Baltimore, Md.) 20130509 7


The granulosa cells in the mammalian ovarian follicle respond to gonadotropin signaling and are involved in the processes of folliculogenesis and oocyte maturation. Studies on gene expression and regulation in human granulosa cells are of interest due to their potential for estimating the oocyte viability and in vitro fertilization success. However, the posttranscriptional gene expression studies on micro-RNA (miRNA) level in the human ovary have been scarce. The current study determined the miR  ...[more]

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