Transcriptomics,Multiomics

Dataset Information

3

Expression data of NIH3T3 in G0 and G1 phases


ABSTRACT: NIH3T3 in the middle of G0 to G1 transion consists of the cells which is still staying G0 phase and the cells which enters G1. Monitoring the expressions of p27 and Cdt1 enables to distinguish these two; p27+/Cdt1+ cells as the cells in G0 phase and p27-Cdt1+ cells as G1 phase We sorted p27+Cdt1+ (G0) NIH3T3 cells and p27-Cdt1+ (G1) NIH3T3 cells in the middle of G0-G1 transition, 5 hours after serum addition following the serum addition using mVenus-p27K- and mCherry-hCdt1(30/120) . We compared the expression profiles of these NIH3T3 cells in G0 and G1 phases and identified the genes upregulated in G0 or G1 phases. The p27+Cdt1+ (G0) NIH3T3 cells and p27-Cdt1+ (G1) NIH3T3 cells were sorted by FACS for RNA extraction and hybridization on Affymetrix microarrays.

OTHER RELATED OMICS DATASETS IN: PRJNA200707

ORGANISM(S): Mus musculus  

SUBMITTER: Atsushi Miyawaki   Kumi Izawa  Akie Maehara  Atsushi Niida  Koutarou Nishimura  Toshihiko Oki  Jiro Kitaura  Satoru Miyano  Katsuhiro Togami  Toshio Kitamura  Hiroyuki Aburatani  Asako Sakaue-Sawano 

PROVIDER: E-GEOD-46511 | ArrayExpress | 2014-02-21

SECONDARY ACCESSION(S): GSE46511PRJNA200707

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-46511.idf.txt Idf
E-GEOD-46511.processed.1.zip Processed
E-GEOD-46511.raw.1.zip Raw
E-GEOD-46511.sdrf.txt Txt
Items per page:
1 - 4 of 4
altmetric image

Publications


The quiescent (G0) phase of the cell cycle is the reversible phase from which the cells exit from the cell cycle. Due to the difficulty of defining the G0 phase, quiescent cells have not been well characterized. In this study, a fusion protein consisting of mVenus and a defective mutant of CDK inhibitor, p27 (p27K(-)) was shown to be able to identify and isolate a population of quiescent cells and to effectively visualize the G0 to G1 transition. By comparing the expression profiles of the G0 an  ...[more]

Similar Datasets

2014-02-21 | E-GEOD-46511 | BioStudies
1000-01-01 | S-EPMC5386458 | BioStudies
2013-01-01 | S-EPMC3774768 | BioStudies
2016-01-01 | S-EPMC4943684 | BioStudies
2014-01-01 | S-EPMC3915272 | BioStudies
2013-01-01 | S-EPMC7654252 | BioStudies
2011-12-01 | E-GEOD-20068 | ArrayExpress
2010-01-01 | S-EPMC2908539 | BioStudies
1000-01-01 | S-EPMC4680797 | BioStudies
2005-01-01 | S-EPMC1201501 | BioStudies