Genomics

Dataset Information

296

The Swi/Snf tumor suppressor complex establishes nucleosome occupancy at target promoters [Mnase-Seq II]


ABSTRACT: Precise nucleosome-positioning patterns at promoters are thought to be crucial for faithful transcriptional regulation. However, the mechanisms by which these patterns are established and dynamically maintained and subsequently contribute to transcriptional control are poorly understood. The Swi/Snf (Baf) chromatin remodeling complex is a master developmental regulator and tumor suppressor that is capable of mobilizing nucleosomes in biochemical assays. Yet, its role in establishing the nucleosome landscape in vivo is unclear. Here we have inactivated Snf5 and Brg1, core subunits of the mammalian Swi/Snf complex, to evaluate their effects on chromatin structure and transcription levels genome-wide. We find that inactivation of either subunit leads to disruptions of specific nucleosome patterning combined with a loss of overall nucleosome occupancy at a large number of promoters, regardless of their association with CpG islands. These rearrangements are accompanied by gene expression changes that promote cell proliferation. Collectively, these findings define a direct relationship between chromatin-remodeling complexes, chromatin structure, and transcriptional regulation. Nucleosome profiling in three different cell types under two digestion conditions

ORGANISM(S): Mus musculus  

SUBMITTER: Michael Y Tolstorukov   Erik J Tillman  Peter J Park  Charles W Roberts  Katherine C Helming  Edward C Koellhoffer  Ping Lu  Courtney G Sansam  Julia A Evans  Burak H Alver  Boris G Wilson 

PROVIDER: E-GEOD-46586 | ArrayExpress | 2013-06-04

SECONDARY ACCESSION(S): SRP022045GSE46586PRJNA201049

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Swi/Snf chromatin remodeling/tumor suppressor complex establishes nucleosome occupancy at target promoters.

Tolstorukov Michael Y MY   Sansam Courtney G CG   Lu Ping P   Koellhoffer Edward C EC   Helming Katherine C KC   Alver Burak H BH   Tillman Erik J EJ   Evans Julia A JA   Wilson Boris G BG   Park Peter J PJ   Roberts Charles W M CW  

Proceedings of the National Academy of Sciences of the United States of America 20130530 25


Precise nucleosome-positioning patterns at promoters are thought to be crucial for faithful transcriptional regulation. However, the mechanisms by which these patterns are established, are dynamically maintained, and subsequently contribute to transcriptional control are poorly understood. The switch/sucrose non-fermentable chromatin remodeling complex, also known as the Brg1 associated factors complex, is a master developmental regulator and tumor suppressor capable of mobilizing nucleosomes in  ...[more]

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