Transcriptomics

Dataset Information

5

Inducible expression of MyoD directly mediates myogenic conversion of human induced pluripotent stem cells (iPSCs) derived from Duchenne muscular dystrophy (DMD).


ABSTRACT: Genome-wide gene expression analysis of MyoD-infected DMD-specific iPSCs (GM05112-M5.1) on days 0 (untreated), day 3 and day 8 post Dox treatment, human primary myoblasts (undifferentiated and as differentiated myotubes), and undifferentiated iPSCs from healthy donors (iPSCs-1 and iPSCs-2). DMD-specific iPSCs were infected with lentivirus expressing MyoD under the control of Tet-inducible promoter and another lentivirus expressing the transactivator. To initiate myogenic differentiation, iPSCs were treated with 1µg/ml Dox. RNA was isolated 0, 3 and 8 days later and gene expression analysis was performed.

ORGANISM(S): Homo sapiens  

SUBMITTER: D L Robbins   Peter Flynn  Ramzey Abujarour  Dave Robbins 

PROVIDER: E-GEOD-46633 | ArrayExpress | 2014-01-09

SECONDARY ACCESSION(S): GSE46633PRJNA201337

REPOSITORIES: GEO, ArrayExpress

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Publications

Myogenic differentiation of muscular dystrophy-specific induced pluripotent stem cells for use in drug discovery.

Abujarour Ramzey R   Bennett Monica M   Valamehr Bahram B   Lee Tom Tong TT   Robinson Megan M   Robbins David D   Le Thuy T   Lai Kevin K   Flynn Peter P  

Stem cells translational medicine 20140106 2


Human induced pluripotent stem cells (iPSCs) represent a scalable source of potentially any cell type for disease modeling and therapeutic screening. We have a particular interest in modeling skeletal muscle from various genetic backgrounds; however, efficient and reproducible methods for the myogenic differentiation of iPSCs have not previously been demonstrated. Ectopic myogenic differentiation 1 (MyoD) expression has been shown to induce myogenesis in primary cell types, but the same effect h  ...[more]

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