Dataset Information


Differentiation of a human gut microbiota in an improved three-stage continuous culture model of the human large intestine under anaerobia generated by the bacteria

ABSTRACT: A three-stage continuous fermentative system was developed to simulate and control physicochemical factors of the gut biology. Inoculation was of each reactor was performed from a human fecal sample which was initially amplified with a batch procedure. Samples from the initial feces, the batch and from the bioreactors media were collected to extract bacterial DNA. 16S PCR amplification was performed to assess the microbial diversity at the family level using the HuGChip. Amplified DNA was purified and labelled with either Cy3 or Cy5 dye and hybridized on the microarray. A 5 chip study was realized, each corresponding to hybridization with 250ng of labelled 16S rRNA gene amplicons from either the initial stool, the batch inoculum or fermentative medium different compartments of the simulated colon (Proximal, Transversal and Distal). Each probe (4441) was synthetized in three replicates.

ORGANISM(S): uncultured bacterium  

SUBMITTER: william tottey   Jean-François Brugère  Pierre Peyret  William Tottey  Nadia Gaci  Monique Alric  David Feria-Gervasio 

PROVIDER: E-GEOD-46677 | ArrayExpress | 2013-05-07



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