Transcriptomics

Dataset Information

6

Expression data from E13.5 Fetal Liver LSK Hes1-GFP positive and E13.5 Fetal Liver LSK Hes1-GFP negative


ABSTRACT: Notch signaling defines a conserved, fundamental pathway, responsible for determination in metazoan development and is widely recognized as an essential component of lineage specific differentiation and stem cell self-renewal in many tissues including the hematopoietic system. Until recently, the majority of studies in the hematopoietic system focused on Notch signaling in lymphocyte differentiation and knowledge of individual Notch receptor roles in early hematopoiesis has been limited due to a paucity of genetic tools available To fate-map Notch receptor expression and pathway activity in the hematopoietic system we used tamoxifen-inducible CreER knock-in mice for individual Notch receptors in combination to a novel Notch reporter strain (Hes1GFP) and a conditional gain of function allele of Notch2 receptor (Rosa-lsl-ICN2). E13.5 Foetal Liver lineage negative, cKit+, Sca1+ cells were sorted from Hes-GFP mice for RNA extraction and hybridization on Affymetrix microarrays

ORGANISM(S): Mus musculus  

SUBMITTER: Philmo Oh   Iannis Aifantis  Camille Lobry 

PROVIDER: E-GEOD-46725 | ArrayExpress | 2013-07-01

SECONDARY ACCESSION(S): GSE46725PRJNA202201

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-46725.idf.txt Idf
E-GEOD-46725.processed.1.zip Processed
E-GEOD-46725.raw.1.zip Raw
E-GEOD-46725.sdrf.txt Txt
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Publications


Accumulating evidence suggests that Notch signaling is active at multiple points during hematopoiesis. Until recently, the majority of such studies focused on Notch signaling in lymphocyte differentiation and knowledge of individual Notch receptor roles has been limited due to a paucity of genetic tools available. In this manuscript we generate and describe animal models to identify and fate-map stem and progenitor cells expressing each Notch receptor, delineate Notch pathway activation, and per  ...[more]

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