Transcriptomics

Dataset Information

2

Gene expression analysis of late exponential vs. stationary phase cultures using a custom designed Pseudo-nitzschia multiseries (Ps-n) cDNA microarray


ABSTRACT: Pseudo-nitzschia multiseries (Ps-n) is a toxigenic marine diatom that produces the neurotoxin, domoic acid. We screened for candidate genes that may be involved in domoic acid production by determining changes in transcript profiles in Ps-n cultures that were in late exponential (low-domoic-acid-producing) vs. stationary (high-domoic-acid-producing) growth states. We also identified a number of candidate reference genes for future RT-qPCR studies, based on their stability in this study. Ps-n RNA was extracted from late exponential and stationary phase cultures. Experiments included one axenic culture, and two non-axenic cultures. Experiments were dye-swapped to account for differences in dye labeling and detection efficiencies: a) the axenic culture experiment included 4 technical replicate arrays (i.e., 2 dye-swapped experiments = 4 total hybridizations), and b) the non-axenic culture experiments each included 6 technical replicate arrays (i.e., 3 dye-swapped experiments = 6 total hybridizations).

ORGANISM(S): Pseudo-nitzschia multiseries  

SUBMITTER: Deborah Robertson   Sean Milton  Jerry Pelletier  Katie Rose Boissonneault  Stephen Bates  David E Housman  KatieRose Boissonneault 

PROVIDER: E-GEOD-46845 | ArrayExpress | 2013-09-08

SECONDARY ACCESSION(S): GSE46845PRJNA202454

REPOSITORIES: GEO, ArrayExpress

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