Transcriptomics

Dataset Information

3

Comprehensive gene expression profile of extrahepatic bile ducts in mice with experimental biliary atresia


ABSTRACT: Newborn Balb/c mice were injected intraperitoneally with 1.5x10^6 fluorescent-forming units (ffu) of type- A Rhesus Rotavirus (RRV) or 0.9% normal saline (NS; control) within 24 hours of birth to induce experimental model of biliary atresia. Extrahepatic bile ducts including gallbladder were microdissected en bloc at 3, 7 and 14 days after RRV or saline injections. GeneChip® Mouse Gene 1.0 ST Array (Affymetrix, CA) were used to screen mRNAs whose expression was differently regulated after RRV challenge compared to normal saline controls. Gene expression profiling: Each experimental conditon contains 3 sets of samples. Each sample consists of two to six extrahepatic bile ducts pooled prior to isolation of total RNA to ensure generation of adequate mRNA to perform quantitative experiments.

ORGANISM(S): Mus musculus  

SUBMITTER: Kazuhiko Bessho   Marepalli Rao  Pranavkumar Shivakumar  Reena Mourya  Anil G Jegga  John McGee  Jorge A Bezerra  Stephanie Walters 

PROVIDER: E-GEOD-46967 | ArrayExpress | 2013-06-01

SECONDARY ACCESSION(S): GSE46967PRJNA203252

REPOSITORIES: GEO, ArrayExpress

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