Transcriptomics

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Transcriptomic analysis of mouse lung tissue exposed to multiwalled carbon nanotubes (Mitsui7)


ABSTRACT: There is great interest in substituting animal with in vitro experimentation in human health risk assessment, but there are rather few comparisons of in vitro and in vivo biological responses to engineered nanomaterials (ENM). We used high-content genomics tools, to compare in vivo pulmonary responses of multiwalled carbon nanotubes (MWCNT) to those in vitro in cultured lung epithelial cells at the global transcriptomic level. Mice were exposed via a single intratracheal instillation to 18, 54 or 162 μg of Mitsui7 and samples were collected at 24 hours post-instillation. This experiment examined the pulmonary transcriptional response of female C57BL/6 mice exposed to Mitsui7 multiwalled carbon nanotubes at three doses: D1 (18 μg), D2 (54 μg), D3 (162 μg), and vehicle control. Each dose group was examined 24 hours post-exposure. Each dose group had 6 biological replicates. There were a total of 22 samples included in the final analysis using a two-color reference design.

ORGANISM(S): Mus musculus  

SUBMITTER: Sabina Halappanavar  Sarah Labib   Andrew Williams    

PROVIDER: E-GEOD-46998 | ArrayExpress | 2015-05-05

SECONDARY ACCESSION(S): GSE46998PRJNA203260

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptomic analysis reveals novel mechanistic insight into murine biological responses to multi-walled carbon nanotubes in lungs and cultured lung epithelial cells.

Søs Poulsen Sarah S   Jacobsen Nicklas R NR   Labib Sarah S   Wu Dongmei D   Husain Mainul M   Williams Andrew A   Bøgelund Jesper P JP   Andersen Ole O   Købler Carsten C   Mølhave Kristian K   Kyjovska Zdenka O ZO   Saber Anne T AT   Wallin Håkan H   Yauk Carole L CL   Vogel Ulla U   Halappanavar Sabina S  

PloS one 20131119 11


There is great interest in substituting animal work with in vitro experimentation in human health risk assessment; however, there are only few comparisons of in vitro and in vivo biological responses to engineered nanomaterials. We used high-content genomics tools to compare in vivo pulmonary responses of multiwalled carbon nanotubes (MWCNT) to those in vitro in cultured lung epithelial cells (FE1) at the global transcriptomic level. Primary size, surface area and other properties of MWCNT- XNRI  ...[more]

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