Genomics

Dataset Information

299

Jarid2, a Polycomb member and target of miR-155, regulates the fates of Th17 and Treg cells


ABSTRACT: Genome-wide analysis of Jarid2, Suz12, and c-Maf binding and H3K27me3 profiling in miR-155 KO and WT Th17 performed by ChIP-seq. We found that Jarid2 and c-Maf is differentially expressed in absence of miR-155 and they compete for binding to the Il22 promoter. We highlight targets of Jarid2 and Suz12 in miR-155 KO Th17 cells that are epigenetically silenced by increased H3K27me3 status. Furthermore, genome-wide analysis through Suz12 ChIP-exo in WT and Jarid2fl/fl;CD4cre Th17 reveals defects in PRC2 recruitment in abscence of Jarid2 that results in derepression of genes in Th17 cells. Thus, one main function of miR-155 is to curb epigenetic silencing by targeting Jarid2. Examination of Jarid2, Suz12, c-Maf binding and H3K27me3 changes in miR-155 KO and WT Th17.

ORGANISM(S): Mus musculus  

SUBMITTER: Gokhul K Kilaru   Thelma M Escobar  Stefan A Muljo  GOKHUL KRISHNA KILARU 

PROVIDER: E-GEOD-47528 | ArrayExpress | 2014-06-01

SECONDARY ACCESSION(S): SRP023466GSE47528PRJNA205981

REPOSITORIES: GEO, ArrayExpress, ENA

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