Transcriptomics

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Gene expression of in vitro cultivated preB cells before and after 8, 16 and 24 hours induction of miR-221


ABSTRACT: PreB cells were analyzed for differences in gene expression before and after the overexpression of miR-221. In order to dissect possible targets for the miR-221, gene expression profiles of preB cells un-induced or induced for the miR-221 expression after 8, 16 and 24 hours were compared. All induction time-points, e.g. after 8, 16 and 24 hours were compared to un-induced preB cells and to each other group. Gene expression profiles of un-induced preB cells, preB cells induced for miR-221 expression after 8, 16, and 24 hours were analyzed using Affymetrix MG 430 2.0 whole genome arrays. Each time-point was performed in triplicates for un-induced preB cells and preB cells induced for miR-221 expression after 8, 16, and 24 hours (12 arrays in total). To obtain genes significantly downregulated upon induction of miR-221, the expression profiles of un-induced preB cells were compared to preB cells activated for 8, 16, or 24 h and were also compared to each other. After total RNA extraction, reverse transcription, cDNA extraction, the biotinylated cRNA was transcribed, fragmented, and 15 µg cRNA hybridized in triplicates for each of the four groups to the 12 GeneChip arrays: Group1, un-induced preB cells, Group2, preB cells induced for miR-221 expression after 8h, Group3, preB cells induced for miR-221 expression after 16h, Group4, preB cells induced for miR-221 expression after 24h.

ORGANISM(S): Mus musculus  

SUBMITTER: Fritz Melchers   Szandor Simmons  Joachim R. Grün  Marko Knoll  Joachim R Grün  Corinne Bouquet 

PROVIDER: E-GEOD-47643 | ArrayExpress | 2013-06-05

SECONDARY ACCESSION(S): GSE47643PRJNA207006

REPOSITORIES: GEO, ArrayExpress

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Publications

miR-221 redirects precursor B cells to the BM and regulates their residence.

Knoll Marko M   Simmons Szandor S   Bouquet Corinne C   Grün Joachim R JR   Melchers Fritz F  

European journal of immunology 20130708 9


Pluripotent hematopoietic stem cells and multipotent myeloid/lymphoid progenitors express miR-221 and miR-222. When Pax5 expression commits these progenitors to monopotent pre-B lymphocytes the two microRNAs (miRNAs) are downregulated. Upon transplantation, stem cells and progenitors can reside in the BM, while pre-B cells, after their commitment, no longer do so. Retrovirally transduced, doxycycline-induced overexpression of either miR-221 or miR-222 in pre-B-I cells does not revert their monop  ...[more]

Publication: 1/2

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