Transcriptomics

Dataset Information

6

Chronic Psychological Stress regulates Visceral Adipocyte-mediated Glucose Metabolism and Inflammatory Circuits in male Rats


ABSTRACT: In this study we employed the chronic unpredictable stress (CUS) rat model that leads to anxiety features comparable to humans and validated in several published reports as a well-characterized model of depression symptoms with high predictive validity. Cytokines and activated intracellular kinase levels were determined using high throughput multiplex assays. RNA from freshly isolated adipocytes was used to run whole genome expression microarray profiling in control and stressed rats. Adipocyte function was assessed via tritiated glucose uptake assay. The expression of four cytokines (TNFα, IL-1β, IL-6 and MCP-1) was validated via real-time PCR and the all showed increased expression levels with chronic unpredictable stress. Male rats were subjected to chronic unpredictable stress for 35 days and total body fat was measured. The analyses presented here represents data from experiments performed in 6 control and 6 stressed rats in parallel. All cells for RNA isolation were collected at the conclusion of the 35 day stress protocol.

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Sylvie Bradesi   Charalabos Pothoulakis  Iordanis Karagiannidis  Iordanes Karagiannides 

PROVIDER: E-GEOD-47754 | ArrayExpress | 2014-06-03

SECONDARY ACCESSION(S): GSE47754PRJNA208050

REPOSITORIES: GEO, ArrayExpress

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Publications

Chronic unpredictable stress regulates visceral adipocyte-mediated glucose metabolism and inflammatory circuits in male rats.

Karagiannides Iordanes I   Golovatscka Viktoriya V   Bakirtzi Kyriaki K   Sideri Aristea A   Salas Martha M   Stavrakis Dimitris D   Polytarchou Christos C   Iliopoulos Dimitrios D   Pothoulakis Charalabos C   Bradesi Sylvie S  

Physiological Reports 20140512 5


Chronic psychological stress is a prominent risk factor involved in the pathogenesis of many complex diseases, including major depression, obesity, and type II diabetes. Visceral adipose tissue is a key endocrine organ involved in the regulation of insulin action and an important component in the development of insulin resistance. Here, we examined for the first time the changes on visceral adipose tissue physiology and on adipocyte-associated insulin sensitivity and function after chronic unpre  ...[more]

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