Transcriptomics

Dataset Information

2

SHAE004: SARS-CoV, SARS-dORF6 and SARS-BatSRBD infection of HAE cultures.


ABSTRACT: HAE cultures were infected with SARS-CoV, SARS-dORF6 or SARS-BatSRBD and were directly compared to A/CA/04/2009 H1N1 influenza-infected cultures. Cell samples were collected at various hours post-infection for analysis. Time Points = 0, 12, 24, 36, 48, 60, 72, 84 and 96 hrs post-infection for SARS-CoV, SARS-dORF6 and SARS-BatSRBD. Time Points = 0, 6, 12, 18, 24, 36 and 48 hrs post-infection for H1N1. Done in triplicate for RNA Triplicates are defined as 3 different wells, plated at the same time and using the same cell stock for all replicates. Time matched mocks done in triplicate from same cell stock as rest of samples. Culture medium (the same as what the virus stock is in) will be used for the mock infections. Infection was done at an MOI of 2 for SARS viruses and an MOI of 1 for H1N1.

ORGANISM(S): Homo Sapiens

SUBMITTER: G L Law   Amy Sims  Jean Chang  Ralph Baric  Michael Katze 

PROVIDER: E-GEOD-47962 | ArrayExpress | 2013-07-31

SECONDARY ACCESSION(S): GSE47962PRJNA208499

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-47962.idf.txt Idf
E-GEOD-47962.processed.1.zip Processed
E-GEOD-47962.raw.1.zip Raw
E-GEOD-47962.raw.2.zip Raw
E-GEOD-47962.raw.3.zip Raw
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