Transcriptomics

Dataset Information

62

Neuropeptides:developmental signals in placode progenitor formation


ABSTRACT: Few families of signaling factors have been implicated in the control of development. Here we identify the neuropeptides nociceptin and somatostatin, a neurotransmitter and neuroendocrine hormone, as a class of developmental signals in chick and zebrafish. We show that signals from the anterior mesendoderm are required for the formation of anterior placode progenitors with one of the signals being somatostatin. Somatostatin controls ectodermal expression of nociceptin and both peptides regulate Pax6 in lens and olfactory progenitors. Consequently, loss of somatostatin and nociceptin signaling leads to severe reduction of lens formation. Our findings not only uncover these neuropeptides as developmental signals, but also identify a long-sought-after mechanism that initiates Pax6 in placode progenitors and may explain the ancient evolutionary origin of neuropeptides, pre-dating a complex nervous system. We used progenitors for anterior and posterior sensory placodes dissected from chick embryos HH5-7; these were either processed immediately or cultured for 5 hrs to hybridise to Affymetrix chick array. We aimed to identify genes that are co regualted with Pax6, a key regulator of lens and olfactory progenitor cells. Pax6 is normally present in anterior, but not posterior placode precursors, but upregulated in both after 5 hrs culture.

ORGANISM(S): Gallus gallus  

SUBMITTER: David Chambers   Nicolas Christophorou  Corinne Houart  Monica Tambalo  Laura Lleras-Forero  Andrea Streit 

PROVIDER: E-GEOD-48116 | ArrayExpress | 2014-04-25

SECONDARY ACCESSION(S): GSE48116PRJNA208948

REPOSITORIES: GEO, ArrayExpress

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Publications

Neuropeptides: developmental signals in placode progenitor formation.

Lleras-Forero Laura L   Tambalo Monica M   Christophorou Nicolas N   Chambers David D   Houart Corinne C   Streit Andrea A  

Developmental cell 20130701 2


Few families of signaling factors have been implicated in the control of development. Here, we identify the neuropeptides nociceptin and somatostatin, a neurotransmitter and neuroendocrine hormone, as a class of developmental signals in both chick and zebrafish. We show that signals from the anterior mesendoderm are required for the formation of anterior placode progenitors, with one of the signals being somatostatin. Somatostatin controls ectodermal expression of nociceptin, and both peptides r  ...[more]

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