Dataset Information


Neonatal antibotic prophylaxis modulates intestinal immunity and prevents necrotizing enterocolitis in preterm neonates

ABSTRACT: Caesarean-delivered preterm pigs were fed 3 d of parenteral nutrition followed by 2 d of enteral formula feeding. Antibiotics (n=11) or control saline (n=13) were given twice daily from birth to tissue collection at d 5. NEC-lesions and intestinal structure, function, microbiology and immunity markers were recorded. We used Affymetrix microarrays to investigate gene expression in intestinal tissues of preterm piglets treated with antibiotics or control saline. Twenty-four preterm piglets were delivered by caesarean section on day 105 of gestation from two healthy sows. All piglets were initially provided with parenteral nutrition via a vascular catheter, combined with small amounts of minimal enteral nutrition. On day three, all parenteral nutrition was stopped and total enteral nutrition was given through an oro-gastric feeding tube. Piglets were allocated into controls ( n=13) and an intervention group receiving oral and systemic broad-spectrum antibiotics ( n=11). To assure high systemic and intra luminal MIC values antibiotics were given both orally and intramuscularly. All antibiotics were given directly after feeding with an oral bolus and control pigs were given corresponding amounts of saline. On day five, all piglets were euthanized, and small intestinal tissue collected.



ORGANISM(S): Sus Scrofa

TISSUE(S): Duodenum, Ileum

SUBMITTER: Imke Mulder   Denise Kelly 

PROVIDER: E-GEOD-48125 | ArrayExpress | 2013-08-21


Dataset's files

Action DRS
E-GEOD-48125.idf.txt Idf Processed Raw
E-GEOD-48125.sdrf.txt Txt
Items per page:
1 - 4 of 4
altmetric image


Antibiotics modulate intestinal immunity and prevent necrotizing enterocolitis in preterm neonatal piglets.

Jensen Michael L ML   Thymann Thomas T   Cilieborg Malene S MS   Lykke Mikkel M   Mølbak Lars L   Jensen Bent B BB   Schmidt Mette M   Kelly Denise D   Mulder Imke I   Burrin Douglas G DG   Sangild Per T PT  

American journal of physiology. Gastrointestinal and liver physiology 20131024 1

Preterm birth, bacterial colonization, and formula feeding predispose to necrotizing enterocolitis (NEC). Antibiotics are commonly administered to prevent sepsis in preterm infants, but it is not known whether this affects intestinal immunity and NEC resistance. We hypothesized that broad-spectrum antibiotic treatment improves NEC resistance and intestinal structure, function, and immunity in neonates. Caesarean-delivered preterm pigs were fed 3 days of parenteral nutrition followed by 2 days of  ...[more]

Similar Datasets

2015-07-15 | E-GEOD-48125 | ExpressionAtlas
| E-GEOD-48125 | BioStudies
2013-08-21 | GSE48125 | GEO
2023-06-03 | GSE223347 | GEO
| S-EPMC5003156 | BioStudies
| S-EPMC4073901 | BioStudies
| S-EPMC7174650 | BioStudies
2023-08-02 | GSE234108 | GEO
| S-EPMC4169518 | BioStudies
2017-01-01 | S-EPMC5445116 | BioStudies