Dataset Information


Genome wide mapping of effects of U6atac knockdown on pre-mRNA splicing

ABSTRACT: This project looks into experimentally identifying all minor introns by knocking down the minor spliceosome's catalytic snRNP, U6atac. Knockdown of U6atac by antisense morpholino followed by examining mRNA splicing by RNA-seq

ORGANISM(S): Homo sapiens  

SUBMITTER: Gideon dreyfuss   Ihab Younis 

PROVIDER: E-GEOD-48263 | ArrayExpress | 2013-08-01



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Minor introns are embedded molecular switches regulated by highly unstable U6atac snRNA.

Younis Ihab I   Dittmar Kimberly K   Wang Wei W   Foley Shawn W SW   Berg Michael G MG   Hu Karen Y KY   Wei Zhi Z   Wan Lili L   Dreyfuss Gideon G  

eLife 20130730

Eukaryotes have two types of spliceosomes, comprised of either major (U1, U2, U4, U5, U6) or minor (U11, U12, U4atac, U6atac; <1%) snRNPs. The high conservation of minor introns, typically one amidst many major introns in several hundred genes, despite their poor splicing, has been a long-standing enigma. Here, we discovered that the low abundance minor spliceosome's catalytic snRNP, U6atac, is strikingly unstable (t½<2 hr). We show that U6atac level depends on both RNA polymerases II and III an  ...[more]

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