Genomics

Dataset Information

4

Expression data of TAGLN-overexpressing RKO human colon cancer cells


ABSTRACT: Transgelin was the top-ranked marker of metastatic potential identified in the comparison of node-positive colorectal cancer (CRC) versus node-negative CRC in our previous study. Transgelin is localized in the nucleus of cultured CRC cells and microRNA-mediated knockdown of TAGLN (the gene encoding transgelin) expression modulates the expression of genes involved in the epithelial-to-mesenchymal transition. We performed gene expression profiling on control and transgelin-overexpressing RKO cells using Affymetrix microarray technology. The plasmid carrying TAGLN was generated using the pcDNA6.2/EmGFP-Bsd/V5-DEST vector (Invitrogen, Carlsbad, CA) and the pDONR221-TAGLN-mut plasmid from a previous study by an LR recombination reaction (http://www.invitrogen.com). After lipofectamine-mediated transfection of the TAGLN-carrying plasmid or the vector-only control plasmid, stable transfectants were selected and cultured in medium containing 25 μg/ml blasticidin followed by RNA extraction and hybridization on Affymetrix microarrays. Six samples were obtained from three independent experiments (technical replicates).

ORGANISM(S): Homo sapiens  

SUBMITTER: Yuan-yuan Fang   Hui-min Zhou  Ying Lin 

PROVIDER: E-GEOD-48998 | ArrayExpress | 2013-07-18

SECONDARY ACCESSION(S): GSE48998PRJNA212543

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-48998.idf.txt Idf
E-GEOD-48998.processed.1.zip Processed
E-GEOD-48998.raw.1.zip Raw
E-GEOD-48998.sdrf.txt Txt
Items per page:
1 - 4 of 4

Similar Datasets

| GSE26238 | GEO
2009-01-01 | S-EPMC2680093 | BioStudies
2010-12-23 | E-GEOD-26238 | ArrayExpress
1000-01-01 | S-EPMC4339693 | BioStudies
2010-01-01 | S-EPMC2935088 | BioStudies
2007-11-11 | E-GEOD-4737 | ArrayExpress
2015-06-12 | E-GEOD-63912 | ArrayExpress
2017-01-01 | S-EPMC5728889 | BioStudies
2015-03-01 | E-GEOD-26998 | ArrayExpress
2009-12-19 | E-MEXP-2396 | ArrayExpress