Transcriptomics

Dataset Information

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Transcription profiling of human MCF10A-Myc breast cancer cell line treated with dexamethazone or an ethanol control, sampled at 4 timepoints


ABSTRACT: This series contain time course microarray data from MCF10A-Myc cells treated with either ethanol or Dexamethasone for 30 min, 2 hr, 4 hr, and 24 hr. This series contains three biological replicates that were analyzed as independent replicate experiments (data were normalized within each replicate experiment, not across all samples). Keywords: time course MCF10A-Myc cells were subjected to 72 hrs of growth factor withdrawal followed by treatment with either vehicle (ethanol) or Dex (10-6 M) for 0.5, 2, 4 or 24 hrs. Total RNA from each sample was extracted using Qiagen’s RNeasy Kit. Preparation of biotinylated cRNA and hybridization to oligonucleotide arrays (Affymetrix human genome genechip HG-U133A) were performed at the University of Chicago Microarray Core Facility. HG-U133A genechip contains 22,215 probe sets that represent approximately 16,000 human genes. The complete procedure was repeated independently three times. Gene chips were scanned and analyzed using Robust Multi-array Average (RMA) algorithm.

ORGANISM(S): Homo sapiens  

SUBMITTER: Suzanne Conzen   Wei Wu  Zou Min  Travis Pew  Deanna Brickley 

PROVIDER: E-GEOD-4917 | ArrayExpress | 2006-06-01

SECONDARY ACCESSION(S): GSE4917PRJNA95859

REPOSITORIES: GEO, ArrayExpress

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Publications

Glucocorticoid receptor activation signals through forkhead transcription factor 3a in breast cancer cells.

Wu Wei W   Zou Min M   Brickley Deanna R DR   Pew Travis T   Conzen Suzanne D SD  

Molecular endocrinology (Baltimore, Md.) 20060511 10


Activation of the glucocorticoid receptor (GR) plays a critical role in the stress response of virtually all cell types. Despite recent advances in large-scale genomic and proteomic data acquisition, identification of physiologically relevant molecular events downstream of nuclear hormone receptor activation remains challenging. By analyzing gene expression changes 30 min after dexamethasone (Dex) treatment, we previously found that immediate induction of serum and glucocorticoid-regulated kinas  ...[more]

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