Transcriptomics

Dataset Information

3

A variant in the neuropeptide receptor npr-1 is a major determinant of Caenorhabditis elegans growth and physiology


ABSTRACT: The mechanistic basis for how genetic variants cause differences in phenotypic traits is often elusive. We identified a quantitative trait locus in C. elegans that affects three seemingly unrelated phenotypic traits: lifetime fecundity, adult body size, and susceptibility to the human pathogen Staphyloccus aureus. We found a QTL for all three traits arises from variation in the neuropeptide receptor gene npr-1. Moreover, we found that variation in npr-1 is also responsive for differences in 247 gene expression traits. Variation in npr-1 is known to determine whether animals disperse throughout a bacterial lawn or aggregate at the edges of the lawn. We found that the allele that leads to aggregation is associated with reduced growth and reproductive output. The altered gene expression pattern caused by this allele suggests that the aggregation behavior might cause a weak starvation state, which is known to reduce growth rate and fecundity. Importantly, we show that variation in npr-1 causes each of these phenotypic differences through behavioral avoidance of ambient oxygen concentrations. These results suggest that variation in npr-1 has broad pleiotropic effects mediated by altered exposure to bacterial food. Two-channel experiment comparing mixed stage sample to mixed stage mixed 50:50 N2,CB4856 common reference

ORGANISM(S): Caenorhabditis elegans  

SUBMITTER: Erik C Andersen   Leonid Kruglyak  Joshua S Bloom 

PROVIDER: E-GEOD-49307 | ArrayExpress | 2013-07-30

SECONDARY ACCESSION(S): GSE49307PRJNA213610

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

| GSE69115 | GEO
2011-08-16 | GSE16765 | GEO
2011-08-15 | E-GEOD-16765 | ArrayExpress
2015-04-23 | E-GEOD-60429 | ArrayExpress
2015-04-23 | E-GEOD-60638 | ArrayExpress
2009-08-05 | GSE17453 | GEO
2018-05-29 | E-MTAB-6407 | ArrayExpress
2003-07-19 | GSE539 | GEO
2014-03-31 | E-GEOD-55400 | ArrayExpress
2007-08-09 | GSE8298 | GEO