Transcriptomics

Dataset Information

63

Mapping of somatic linker histone H1 variants in T47D-MTVL and HeLa cells


ABSTRACT: At least six histone H1 variants exist in mammalian somatic cells that bind to the linker DNA and stabilize the nucleosome particle contributing to higher order chromatin compaction. In addition, H1 seems to be involved in the active regulation of gene expression. It is not well known whether the different variants have specific roles, are distributed differentially along the genome, or regulate specific promoters. By taking advantage of specific antibodies to H1 variants and HA-tagged recombinant H1 variants expressed in a breast cancer-derived cell line, we have investigated the distribution of the different somatic H1 variants (H1.2 to H1.5, H1.0 and H1X) in particular promoters and genome-wide. Analysis of H1 (H1.0, H1.2, H1.3, H1.4, H1.5 and H1X) and H3 abundance in promoter regions

ORGANISM(S): Homo sapiens  

SUBMITTER: Lluís Millán-Ariño   Albert Jordan 

PROVIDER: E-GEOD-49333 | ArrayExpress | 2014-02-04

SECONDARY ACCESSION(S): GSE49333PRJNA213642

REPOSITORIES: GEO, ArrayExpress

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Publications

Mapping of six somatic linker histone H1 variants in human breast cancer cells uncovers specific features of H1.2.

Millán-Ariño Lluís L   Islam Abul B M M K AB   Izquierdo-Bouldstridge Andrea A   Mayor Regina R   Terme Jean-Michel JM   Luque Neus N   Sancho Mónica M   López-Bigas Núria N   Jordan Albert A  

Nucleic acids research 20140129 7


Seven linker histone H1 variants are present in human somatic cells with distinct prevalence across cell types. Despite being key structural components of chromatin, it is not known whether the different variants have specific roles in the regulation of nuclear processes or are differentially distributed throughout the genome. Using variant-specific antibodies to H1 and hemagglutinin (HA)-tagged recombinant H1 variants expressed in breast cancer cells, we have investigated the distribution of si  ...[more]

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