Transcriptomics

Dataset Information

9

Differentiation of human amniotic fluid kidney progenitor cells into podocytes and comparison with human conditionally immortalized podocytes


ABSTRACT: In this work, we isolated and characterized a novel cell population derived from human amniotic fluid cells (hAKPC-P), and we differentiated them into podocytes. We used microarrays to study global changes in gene expression before and after differentiation in hAKPC-P and human immortalized podocytes (hIPod, positive control) and performed a detailed comparison between the different populations hAKPC-P were isolated by FACS sorting from the total human amniotic fluid cell population and differentiated into podocytes using VRADD media. Morphological, phenotypical and functional analysis were performed to assess their differentiation. To confirm the results, cells were compared with human conditionally immortalized podocytes.

REANALYSED by: E-GEOD-49439

ORGANISM(S): Homo sapiens  

SUBMITTER: Stefano Da Sacco   Laura Perin 

PROVIDER: E-GEOD-49439 | ArrayExpress | 2013-12-19

SECONDARY ACCESSION(S): GSE49439PRJNA213912

REPOSITORIES: GEO, ArrayExpress

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Publications


Amniotic fluid is in continuity with multiple developing organ systems, including the kidney. Committed, but still stem-like cells from these organs may thus appear in amniotic fluid. We report having established for the first time a stem-like cell population derived from human amniotic fluid and possessing characteristics of podocyte precursors. Using a method of triple positive selection we obtained a population of cells (hAKPC-P) that can be propagated in vitro for many passages without immor  ...[more]

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