Dataset Information


Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub

ABSTRACT: The aim of the dataset was to study on a genome-wide level the impact of Lat deficiency on gene expression in resting and activated CD4+ T cells Lat+ and Lat− CD4+ T cells were isolated from lymph nodes and spleen of Latfl-dtr Tmat-Cre mice using a Dynabeads untouched mouse CD4 cells kit (Life Technology) and further purified by cell sorting. Lat+ CD4+ T cells were defined as: CD5+, hDTR+, CD8−, TCRγδ−, CD25−, CD69−, CD62L+, lineage (CD11c, CD11b, CD19, CD45R, CD161)− and Lat− CD4+ Tcells were defined as: CD5+, hDTR−, CD8−, TCRγδ−, CD25−, CD69−, CD62L+, lineage (CD11c, CD11b, CD19, CD45R, CD161)−. Sorted Lat+ or Lat− CD4+ T cells were then kept in vitro for 4 hours without stimulation or activated for 4 hours using anti-CD3 antibody and anti-CD28 antibody. Cell samples corresponding to three biological replicates were analyzed and gene expression profiles were obtained from total RNA.

ORGANISM(S): Mus musculus  

SUBMITTER: Ruedi Aebersold   Aurelie Malzac  Harri Lähdesmäki  Bernard Malissen  Romain Roncagalli  Marie Malissen  Fréderic Fiore  Amandine Sansoni  Riitta Lahesmaa  Kartiek Kanduri  Matthias Gstaiger  Simon Hauri  Zhi Chen  Yinming Liang  Sho Yamasaki  Rachel Joly  Takashi Saito 

PROVIDER: E-GEOD-49507 | ArrayExpress | 2014-03-02



Similar Datasets

2012-02-10 | E-MEXP-2578 | ArrayExpress
| GSE119372 | GEO
2011-07-31 | E-GEOD-23663 | ArrayExpress
2011-08-01 | GSE23663 | GEO
2020-03-11 | E-MTAB-8862 | ArrayExpress
2009-09-14 | GSE15246 | GEO
2013-06-14 | E-GEOD-47909 | ArrayExpress
2009-09-14 | E-GEOD-15246 | ArrayExpress
2015-08-05 | E-GEOD-67884 | ArrayExpress
2017-08-15 | E-MTAB-4853 | ArrayExpress