Transcriptomics

Dataset Information

4

2D and 3D Madin-Darby Canine Kidney Cell Type II cell line response to hepatocyte growth factor


ABSTRACT: Analysis of 2D (transwell) and 3D (collagen type I) cultured MDCK cells and HGF (a MAPK activator). Traditional 2D cultures are fast and inexpensive but do no mimic natural niche/cell environment as well as the more laborious and costly 3D-cultures. 3D cultures, arguably, are better models for the study of developmental processes, such as tubulogenesis. Epithelial organs (such as kidney) develop via tubulogenesis, a process, at least in part, regulated by MAPK signaling. Therefore, 2D and 3D cells also treated with HGF plus MAPK inhibitors. Results provide insights into differential response to HGF-induced tubulogenesis depending on cell culture conditions (2D vs. 3D). 29 samples total: 2D and 3D control (untreated) in quadruplicate, respectively; 2D and 3D + HGF in quadruplicate, respectively; 2D + HGF + PD-98059 in quadruplicate; 3D + HGF + PD-98059 in triplicate; 2D + HGF + U0126 in triplicate; and 3D + HGF + U0126 in triplicate.

ORGANISM(S): Canis lupus familiaris  

SUBMITTER: Maria F Chacon-Heszele   Xiaofeng Zuo  Nathan E Hellman  Soo Y Choi  Kwon M Park  Sarah McKenna  John W Tobias  Liwei Huang  Maria Fernanda Chacon-Heszele  Joshua H Lipschutz 

PROVIDER: E-GEOD-49518 | ArrayExpress | 2014-03-31

SECONDARY ACCESSION(S): GSE49518PRJNA214098

REPOSITORIES: GEO, ArrayExpress

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