Transcriptomics

Dataset Information

36

The regulation of miRNA expression by DNA methylation in hepatocellular carcinoma


ABSTRACT: Emerging evidences indicate that microRNAs (miRNAs) are often deregulated and have fundamental roles in hepatocellular carcinoma (HCC). However, the mechanism underlying miRNA dysregulation in HCC is still elusive. In this report, we used an integrated analysis strategy combining methylated DNA immunoprecipitation chip (MeDIP-chip) and miRNA expression microarray data to study the correlation between aberrant methylation and dysregulation of miRNA in HCC. In all, we showed that global miRNA methylation profiles were significantly different between cancerous and normal hepatocytes, and abnormal methylation was an important mechanism governing miRNA expression in HCC. MeDIP-chip was processed in cancerous hepatocytes SK-HEP-1, HepG2, MHCC97-H and normal hepatocytes PHHC-4-1, PHHC-4-2, PHHC-4-3 (3 technical repeat of PHHC-4). MiRNA microarray were processed for cancerous hepatocytes SK-HEP-1, HepG2, Hep3B, Huh7, MHCC97-H, MHCC97-L, SMMC-7721 and normal hepatocytes PHHC-1, PHHC-2, PHHC-3. Then an integrated analysis strategy combining MeDIP-chip and miRNA expression microarray [GSE20077] were used to study the correlation of aberrant DNA methylation and dysregulation of miRNAs.

ORGANISM(S): Homo sapiens  

SUBMITTER: Ju-Sheng Lin  Ying Chang   An-Yuan Guo   Xing-Xing He   Shu-Zhen Kuang    

PROVIDER: E-GEOD-49554 | ArrayExpress | 2015-08-19

SECONDARY ACCESSION(S): GSE49554PRJNA214358

REPOSITORIES: GEO, ArrayExpress

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Publications

The regulation of microRNA expression by DNA methylation in hepatocellular carcinoma.

He Xing-Xing XX   Kuang Shu-Zhen SZ   Liao Jia-Zhi JZ   Xu Chuan-Rui CR   Chang Ying Y   Wu Yu-Liang YL   Gong Jing J   Tian De-An DA   Guo An-Yuan AY   Lin Ju-Sheng JS  

Molecular bioSystems 20141126 2


Emerging evidence indicates that microRNAs (miRNAs) are often dysregulated and play a fundamental role in hepatocellular carcinoma (HCC). However, the mechanism underlying miRNA dysregulation is still elusive. In the present study, we adopted an integrated analysis strategy combining data from genome-wide methylated DNA immunoprecipitation chip and miRNA expression microarray to study the regulation of DNA methylation on miRNA expression in HCC. We first characterized 864 differentially methylat  ...[more]

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