Transcriptomics

Dataset Information

3

Promiscuous gene expression in human medullary thymic epithelial cell subsets


ABSTRACT: Different human mTEC subsets (MUC1, CEACAM5 and SGLT1) were purified by sequential enzymatic digestion (collagenase/dispase, trypsin) followed by enrichment using magnetic beads (CD45 beads, Miltenyi Biotech) and FACS sorting. Cells of the surface phenotype CD45-, CDR2-, EpCAM+ were further subdivided into MUC1+/MUC1-, CEACAM5+/CEACAM5- and SGLT1+/SGLT1- fractions. RNA was isolated using μMACS™ SuperAmp™ protocol (Miltenyi Biotec) and hybridized to Illumina Whole-Genome Expression Beadchips. Gene expression of Antigen-positive and Antigen-negative mTEC subsets was compared. Total RNA was isolated from ex-vivo isolated human mTEC subsets using μMACS™ SuperAmp™ protocol (Miltenyi Biotec)

ORGANISM(S): Homo sapiens  

SUBMITTER: C Michel   H Schmidt-Glenewinkel  S Wild  S Pinto  Benedikt Brors  B Kyewski  B Brors 

PROVIDER: E-GEOD-49625 | ArrayExpress | 2013-08-31

SECONDARY ACCESSION(S): GSE49625PRJNA214510

REPOSITORIES: GEO, ArrayExpress

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Publications

Overlapping gene coexpression patterns in human medullary thymic epithelial cells generate self-antigen diversity.

Pinto Sheena S   Michel Chloé C   Schmidt-Glenewinkel Hannah H   Harder Nathalie N   Rohr Karl K   Wild Stefan S   Brors Benedikt B   Kyewski Bruno B  

Proceedings of the National Academy of Sciences of the United States of America 20130826 37


Promiscuous expression of numerous tissue-restricted self-antigens (TRAs) in medullary thymic epithelial cells (mTECs) is essential to safeguard self-tolerance. A distinct feature of promiscuous gene expression is its mosaic pattern (i.e., at a given time, each self-antigen is expressed only in 1-3% of mTECs). How this mosaic pattern is generated at the single-cell level is currently not understood. Here, we show that subsets of human mTECs expressing a particular TRA coexpress distinct sets of  ...[more]

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